(Go here for single 2D plane calibrations)
Matlab program to calculate the transformation required to map 2P imaging space onto SLM space, allowing correct targets of ROIs.
- Optional: Calibrate uncaging galvos using zero order spot. This will ensure the centre of SLM space is the centre of 2P imaging space.
- Burn multiple spots onto fluorescent slide (simultaneously or sequentially)
- take a 2P volume stack of the burnt slide
- Register the SLM targets image and the 2P burnt spot image
- Use the saved transform when making all future SLM phase masks
