nf-core/funcscan

Missing Annotation files

Closed this issue · 2 comments

Description of the bug

I encountered an issue while running the nf-core/funcscan pipeline, where the expected annotation files (setting Prokka as the annotation tool) were not generated despite the pipeline completing its execution. It only generates the multiQC files:
Screenshot from 2024-06-27 16-47-21

Where custom is the custom.config file I created:
Screenshot from 2024-06-27 16-49-02

Command used and terminal output

nextflow run nf-core/funcscan -r 1.1.0 -profile custom,docker --outdir results/ -c custom.config

Relevant files

No response

System information

No response

I believe this is because annotation will not run unless one of the subworkflows are turned on.

Currently they are all turned off because by default each is set to not run unless you turn it on, and you have just set the same setting in your config.

I presume you want to run the BGC subworkflow. Si make sure to set --run_bgc_screening in your command.

Please also note that setting pipeline settings in the configs are not recommended, rather use a params yaml file (-params-file).

@jfy133 Thank you for your guidance. Following your advice, I ensured that the BGC subworkflow is set to run by including --run_bgc_screening in my command together with the parameter --save_annotations to save the annotations results in the output directory. It worked perfectly!

Thanks again for your help!