Using a 5' PAM for enumeration
ericmalekos opened this issue · 0 comments
ericmalekos commented
Thank you for the great tool.
Is there anyway to enumerate off-targets under the assumption that that the PAM is 5' to the protospacer?
For Cas12A for example the targets are 5' - PAM (TTTV) - protospacer - 3'