Ambiguous bases
OliverPStuart opened this issue · 1 comments
OliverPStuart commented
Hi there,
I am examining a wtdbg2.5 assembly and I have two contigs with short (23 bp) stretches of ambiguous N bases. This is not a problem per se but I do not understand what these Ns represent.
Can you please advise?
Best,
Ollie
ruanjue commented
Possiblly caused by mis-joint of seqs of nearby edges, but this bug is too rare to be debuged.