barcode setting
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Jarvis559 commented
Hi y9c!
I have a question here. I use the R2 reads for your pipeline，and there are 8nt UMI and 6nt random sequence in the 5' end, and i have already trimmed the 3' end adapter, how should i set the barcode, is "NNNNNNNNXXXXXX-" work? Looking forward to your reply, thanks!
y9c commented
Hi @Jarvis559, Yes. "NNNNNNNNXXXXXX-" should work. You can test it and if there is any issue, please let me know. Thank you.