Issue with execution of the Ribo-ODDR tool
Closed this issue · 4 comments
I am trying to design rRNA depletion probes and I tried to use the example data with mouse_rRNA.fa as -r input and then placed the output in the current directory
it gives this error
Traceback (most recent call last):
File "src/Ribo-ODDR.py", line 495, in
main()
File "src/Ribo-ODDR.py", line 317, in main
if len(args.samples)<1:
TypeError: object of type 'NoneType' has no len()
Can you please explain whats going on ?
Hi there. Seems like there is a problem with how you pass your input files to the pipeline. Can you share your full command so that I can help?
This is the command I placed in terminal
Ribo-ODDR-master/src/Ribo-ODDR.py -r DATA/Ribosomal_RNA -o /Results
Ribo-ODDR cannot design oligos without any pilot Ribo-seq data. It is made for designing oligos in an experiment-specific manner, with pilot data provided by the user for the specific experimental conditions. In your command line, you are not passing any pilot data to the pipeline, therefore it fails to execute. Please take a look at our README file to see how you can pass your pilot data to the pipeline.
Yes, thank you