Missing rMATS arguments
adomingues opened this issue · 2 comments
adomingues commented
Description of the bug
Not a bug as such, but more of a missing argument which is critical for rMATS.
one of the key rMATS arguments is libType
(https://github.com/Xinglab/rmats-turbo?tab=readme-ov-file#all-arguments):
--libType {fr-unstranded,fr-firststrand,fr-secondstrand}
Library type. Use fr-firststrand or fr-secondstrand
for strand-specific data. Only relevant to the prep
step, not the post step. Default: fr-unstranded
however in the rnasplice docs I don't see it exposed in the pipeline. Is is possible to choose the library type and I just missed?
Command used and terminal output
No response
Relevant files
No response
System information
No response
jma1991 commented
Hello @adomingues
The library type parameter is inferred based on the value of the strandedness column in the input sample sheet file. See here.
If this solves your query, I will go-ahead and close the issue.
adomingues commented
Thanks @jma1991, that answers my question perfectly.