primer3-org/primer3

Issues

• Understanding the dpal logic

  #70 opened 2 years ago by karen-nativ
  1

• Penalty for template pair mispriming is perhaps calculated twice

  #63 opened 3 months ago by Tarasovk49
  1

• Abnormal primer positions

  #71 opened 4 months ago by shiyi-pan
  3

• ntthal set defaults are different than ntthal usage information defaults

  #79 opened 4 months ago by emmcauley
  1

• SEQUENCE_PRIMER_PAIR_OK_REGION_LIST doesn't honor specified values

  #81 opened 4 months ago by kubu4
  1

• Issue when make test

  #75 opened 4 months ago by nanu4github
  1

• ntthal negative Tm calculations

  #77 opened 4 months ago by cesimsek
  2

• Question: How PRIMER_MISPRIMING_LIBRARY works?

  #74 opened 2 years ago by harazono
  0

• A question about picking sequencing primers

  #72 opened 2 years ago by yangjw1996
  2

• `max_loop` not working when calculating hairpin structure?

  #73 opened 2 years ago by dodoflyy
  2

• Question: Is ARM64 architecture supported ?

  #68 opened 3 years ago by julien-faye
  2

• What PRIMER_PRODUCT_OPT_SIZE is needed for?

  #67 opened 3 years ago by DmitriiSukhomlinov
  2

• Signal 11 segmentation fault in thal.c

  #65 opened 3 years ago by InvisOn
  2

• A question about ntthal to cacualte "ANY" bounds between two oligos

  #64 opened 3 years ago by zenghp88
  0

• Final record not terminated by '='

  #62 opened 3 years ago by Miduoduoduoduomi
  4

• unable to open file /opt/primer3_config/stack.ds

  #61 opened 3 years ago by Hytham79
  7

• Scoring pre-selected primers?

  #59 opened 3 years ago by joshuak94
  3

• Segmentation fault

  #60 opened 3 years ago by Princysaini
  1

• critical memory error

  #58 opened 3 years ago by jocelyne8
  4

• When is the next release of Primer3

  #48 opened 3 years ago by nh13
  3

• critical memory error with humrep_and_simple.txt as mispriming library

  #57 opened 3 years ago by jocelyne8
  3

• Could primer3 design primers based on alignment

  #56 opened 3 years ago by starsareintherose
  3

• thal.c caused crash of build on ubuntu, "/0" needs to be just zero

  #55 opened 3 years ago by sebogawa
  1

• thal.c caused crash of build on ubuntu, "/0

  #54 opened 3 years ago by sebogawa
  2

• how to determine the integer input for SEQUENCE_OVERLAP_JUNCTION_LIST

  #53 opened 3 years ago by juechenyang
  3

• Possible BUG: No triloop and tetraloop bonuses for entropy?

  #52 opened 3 years ago by Oleg-Mozhey
  1

• Shouldn't stackmm value for TA_AA be 4700 instead of inf?

  #35 opened 5 years ago by Curley-Joe
  2

• The salt ion correction formula in file thal.c is inconsistent with that in file oligotm.c

  #36 opened 3 years ago by Wy2160640
  1

• Primer3 not finding microsatellite primers from reference genome

  #37 opened 3 years ago by ctdarwell
  1

• What is the difference between primer3web and primer3Plus ?

  #50 opened 3 years ago by ptranvan
  1

• Primer3 issue with the Sequence_ID

  #51 opened 3 years ago by Kisekya
  3

• Crash in check_primers if primers are perfect match

  #33 opened 3 years ago by Curley-Joe
  3

• Feature request: Multiple structures from thal

  #30 opened 3 years ago by Permafacture
  6

• number of 'ok' primers is sometimes negative

  #41 opened 3 years ago by wholtz
  1

• number of warnings building with clang on macOS

  #22 opened 3 years ago by nivekkagicom
  1

• Multiplexing assays takes a lot of time

  #45 opened 3 years ago by haseebz
  2

• Stray text NOWWW in manual

  #46 opened 3 years ago by peterjc
  1

• Return all primers for pick_sequencing_primers task

  #32 opened 3 years ago by shunhuahan
  1

• Question on calcHairpin dg discrepancy

  #38 opened 3 years ago by sooheelee
  3

• How to set the SEQUENCE_TARGET or SEQUENCE_EXCLUDED_REGION parameter?

  #47 opened 3 years ago by sen1019san
  1

• poly_x calculation failures

  #39 opened 3 years ago by wholtz
  0

• Nested structure for primer results

  #44 opened 4 years ago by peterjc
  1

• Question re TEMPLATE_MISPRIMING parameters

  #43 opened 4 years ago by genya
  1

• primer-blast setting file ?

  #21 opened 5 years ago by ptranvan
  2

• How to connect primer 3 with emboss 6.6.0

  #28 opened 5 years ago by aslangabriel
  1

• How to exclude specific target regions?

  #27 opened 6 years ago by billzt
  2

• Link to manual in README

  #26 opened 6 years ago by simone-pignotti
  2

• problem installing on Win10

  #25 opened 6 years ago by liucyuic
  1

• ntthal alignment not complete. Incomplete thermodynamic calculation?

  #24 opened 6 years ago by Permafacture
  2

• The oligotm tool has been outdated

  #23 opened 6 years ago by billzt
  1