Edinburgh-Genome-Foundry/DnaCauldron

Issues

• Palindromes in Golden Gate assembly, only want one construct

  #22 opened 6 months ago by stephenturner
  5

• Assembling fragments digested by different enzymes

  #15 opened 2 years ago by AubinF
  5

• Unwanted case-sensitivity when indexing fragment against source record

  #20 opened a year ago by bwvogler
  2

• TypeError: StickyEndSeq.reverse_complement() got an unexpected keyword argument 'inplace'

  #18 opened 2 years ago by antonkulaga
  1

• Purpose of OligoPairAnnealing class (oligo_annealing method in assembly plan)

  #14 opened 2 years ago by veghp
  2

• example with ladders

  #17 opened 2 years ago by antonkulaga
  1

• Test fails with Biopython v1.79

  #16 opened 2 years ago by veghp
  1

• annotations crossing overhangs lost

  #8 opened 4 years ago by aaroncooper
  7

• Linear assemblies?

  #5 opened 4 years ago by gjerman
  3

• Annotating features with qualifiers

  #13 opened 3 years ago by benweinberg89
  1

• Check parts assembling into enzyme sites

  #10 opened 3 years ago by veghp
  0

• pdf_reports required but not installed by pip

  #11 opened 3 years ago by fbertaux
  1

• The tutorial uses parts as keyword argument for SequenceRepository but now its collections as dict of dict

  #12 opened 3 years ago by fbertaux
  1

• Choosing enzyme in AssemblyPlan.from_spreadsheet()

  #9 opened 3 years ago by veghp
  0

• problem with snapgene_reader

  #2 opened 5 years ago by jurquiza
  3